Insights from the GC content analysis of 76 genome survey sequences (GSS) from Elaeis oleifera



Subhash J. Bhore1,2,3*, Amelia Kassim1,4, Farida H. Shah1,2



1Molecular Biology Division, Melaka Institute of Biotechnology, Lot 7, Melaka International Trade Center City, 75450 Ayer Keroh, Melaka, Malaysia; 2School of Biosciences and Biotechnology, National University of Malaysia, Selangor, Malaysia;3Current Address: Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong-Semeling Road, Bedong, 08100, Kedah, Malaysia;4Current Address: Faculty of Information Science and Technology, Multimedia University, Jalan Ayer Keroh Lama, 75450, Melaka, Malaysia;

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received August 19, 2010; accepted August 26, 2010; published September 20, 2010


South American oil-palm (Elaeis oleifera) is not cultivated in tropical countries like Malaysia on large scale due to low yield of palm oil derived from its fruit mesocarp. However, its fruit mesocarp oil contains about 68.6 % oleic acid (C18:1) which is more than double in comparison to commercially cultivated oilpalm, E. guineensis Jacq Tenera (hybrid of Dura (?) x Pisifera (?)). It is also known that E. oleifera is a good source of tocotrienols and carotenoids. Therefore, it is of interest to know the genome sequence of E. oleifera. The objective of this study is to generate genome survey sequences (GSS) to get GC content insight in the E. oleifera genome. The nuclear genomic DNA isolated from young leaf-tissues was digested with EcoRI and NdeI/DraI restriction enzymes; and three genomic DNA libraries were constructed using Lambda ZAP-II, pGEMŪ-T Easy, and pDONR 222TM as cloning vectors. Generated 76 GSSs were analyzed by using Bioinformatics tools. The analysis result indicates that the adenine, cytosine, guanine and thymine content in generated GSSs are 30%, 20%, 20%, and 30% respectively. In conclusion, based on the precise GC content analysis of the randomly isolated 76 GSSs by using Bioinformatics tools we hypothesize that GC content in E. oleifera genome is 40%. The hypothesized 40% GC content in E. oleifera genome is expected to remain close to the GC content based on the whole genome analysis.




American oil-palm, GC content, Genomics, Genome, GSS, in silico, palm oil


Bhore et al. Bioinformation 5(4): 141-145 (2010)


Edited by

P. Kangueane






Biomedical Informatics



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